2 edition of Non-invasive molecular imaging of an EGFP and DRD2-expressing vaccinia virus. found in the catalog.
Non-invasive molecular imaging of an EGFP and DRD2-expressing vaccinia virus.
Written in English
As oncolytic vectors for cancer therapy come to clinical trials, noninvasive monitoring of vector biodistribution will be necessary. Oncolytic vaccinia viruses (rVV) preferentially target, infect and replicate in tumours. The goal of this research was to develop non-invasive strategies of monitoring rVV in vivo. Upon subcutaneous murine colon cancer tumour formation and subsequent systemic administration of rVV in athymic mice, we acquired whole-body mouse images by nuclear medicine or optical (fluorescence) techniques. Fluorescence imaging revealed the earliest time-point of enhanced green fluorescent protein (EGFP) detection and persistence in tumours of mice infected with an EGFP-expressing rVV. In vitro binding studies confirmed specific radioligand binding of 111In-pentetreotide and [123I]IBF to vaccinia virus-mediated expression of human somatostatin receptor subtype 2 (hSSTR2) and the dopamine D2 receptor (DRD2) on tumour cells, respectively. In vivo studies examined biodistribution and specificity of 111In-Pentetreotide and [123I]IBF in athymic mice after systemic delivery of vvDD-hSSTR2 and vvDD-EGFP-DRD2, respectively. Optical and nuclear imaging of rVV expressing EGFP, hSSTR2, and DRD2 provides a non-invasive assessment of vector biodistribution and persistence, and potentially will be able to image tumour responses.
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A gene vector space, or vector database, is a set of vectors X ij = (x i1, x i2, , x iN), where I =1, , M, and j = 1, , N. M and N indicate, respectively, the number of genes and the number of data points/experimental conditions produced by a genomewide experiment and associated with each gene. Measurements may be relative, as with data obtained in two-color printed gene expression. Detection of EGFR mutations in tumour tissue is the gold-standard approach to ascertain if a patient will benefit from treatment with an EGFR tyrosine kinase inhibitor. However, if tissue is scant, another strategy is to use circulating tumour DNA (ctDNA), but this method needs validation in clinical trials.
Investigation of intervertebral disc degeneration using multivariate FTIR spectroscopic imaging†. Kerstin T. Mader * a, Mirte Peeters bc, Suzanne E. L. Detiger bc, Marco N. Helder bc, Theo H. Smit bc, Christine L. Le Maitre d and Chris Sammon a a Sheffield Hallam University, Materials and Engineering Research Institute, Sheffield, S1 1WB, UK. Activation of platelet-derived growth factor receptor alpha (PDGFRA) by genomic aberrations contributes to tumor progression in several tumor types. In this study, we characterize 16 novel PDGFRA mutations identified from different tumor types and identify three previously uncharacterized activating mutations that promote cell survival and proliferation. PDGFRA YC, an extracellular domain.
This is a Phase I clinical trial evaluating crenolanib (CP,), an inhibitor of Platelet Derived Growth Factor Receptor (PDGFR)-kinase in children and young adults with newly diagnosed diffuse intrinsic pontine glioma (DIPG) (Stratum A) or in recurrent, progressive or refractory High Grade Glioma (HGG) including DIPG (Stratum B). The labeling was achieved through the non-covalent interaction of glutathione-capped CdTe quantum dots with the virus envelope, without the use of chemical conjugation. The quantum dot labeling was nondestructive to viral transduction function and enabled the identification of baculoviral vector-transduced, living cells based on red fluorescence.
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The creation of a vaccinia virus vector which will both treat tumours and allow non-invasive imaging is a novel and exciting concept. Molecular Imaging of an EGFP-DRD2-Expressing Vaccinia. Modulation of the cytokine milieu is one approach for vaccine development.
However, therapy with pro-inflammatory cytokines, such as IL, is limited in practice due to adverse systemic effects. Spatially-restricted gene expression circumvents this problem by enabling localized amplification. Intracellular co-delivery of gold nanorods (AuNR) and a heat shock protein 70 (HSP70) promoter-driven Cited by: Mutation details: This transgene contains the coding sequence for enhanced green fluorescent protein (EGFP), followed by a polyadenylation signal, inserted into the mouse genomic bacterial artificial chromosome (BAC) RPH15 at the ATG transcription initiation codon of the dopamine receptor 2 (Drd2) gene so that expression of the reporter mRNA/protein is driven by the.
imaging of rVV expressing EGFP and DRD2 provides a non-invasive assessment of vector biodistribution and persistence, and potentially will be able to image tumour responses.
Thus, we feel oncolytic vaccinia virus is a promising vector for cancer therapy. New Strategy To Improve Imaging and Retargeting of HSV-1 Vectors. Molecular imaging can be defined as “the non-invasive visualization of molecular processes.” Molecular imaging uses X-ray, CT, MRI, and US in addition to Xuorescence microscopy and endoscopy as well as nuclear scanning techniques like positron-emission tomography (PET) and single-photon emission tomography (SPECT).Cited by: 3.
The enhanced green fluorescent protein (EGFP) is widely used in molecular imaging approaches, together with optical scanners, and fluorescence imaging. it is the aim of this book chapter to. Photoacoustic (optoacoustic) imaging can extract molecular information with deeper tissue penetration than possible by fluorescence microscopy techniques.
However, there is currently still a lack of robust genetically controlled contrast agents and molecular sensors that can dynamically detect biological analytes of interest with photoacoustics.
Plasmid pEGFP-N-Drd1 from Dr. Kirk Mykytyn's lab contains the insert Dopamine receptor D1 (Drd1) and is published in Cell Mol Life Sci. Sep;68(17) doi: /s Epub Dec 9. This plasmid is available through Addgene.
Plasmid GFP-DRD2 from Dr. Jean-Michel Arrang's lab contains the insert DRD2 and is published in Mol Biol Cell. Feb. 15(2) This plasmid is available through Addgene.
Chikungunya virus (CHIKV), a member of the Alphavirus genus, is an important human emerging/re-emerging pathogen. Currently, there are no effective antiviral drugs or vaccines against CHIKV infection.
Herein, we construct an infectious clone of CHIKV and an eGFP reporter CHIKV (eGFP-CHIKV) with an isolated strain (assigned to Asian lineage) from CHIKV-infected patients. The term molecular imaging can be broadly defined as the in vivo characterization and measurement of biologic processes at the cellular and molecular level.
In contradistinction to “classical” diagnostic imaging, it sets forth to probe the molecular abnormalities that are the basis of disease rather than to image the end effects of these.
The number of patients with end-stage renal disease, and the number of kidney allograft recipients continuously increases. Episodes of acute cellular allograft rejection (AR) are a negative prognostic factor for long-term allograft survival, and its timely diagnosis is crucial for allograft function present, AR can only be definitely diagnosed by core-needle biopsy, which, as an invasive.
Uncovering the mechanisms of virus infection and assembly is crucial for preventing the spread of viruses and treating viral disease. The technique of single-virus tracking (SVT), also known as single-virus tracing, allows one to follow individual viruses at different parts of their life cycle and thereby provides dynamic insights into fundamental processes of viruses occurring in live cells.
Traditional nuclear imaging has limitations depending on the modality utilized which include poor spatial resolution, low sensitivity, ionizing radiation, or lack of 3D anatomic context. Multimodality imaging techniques integrating anatomical visualization with molecular imaging, such as SPECT/CT and PET/CT are capable of 3D nuclear imaging.
Dacie and Lewis Practical haematology. Twelfth edition. [12th ed.] — A Detailed chronological record of project and the discovery and development of qinghaosu (artemisinin) () Detecting and characterizing large-scale human brain networks — Diagnostic ultrasound imaging: inside out.
Multiplexed FRET for imaging cell signaling and high speed optically sectioned FLIM for high throughput screening applications. Abstract D M Grant1, W Zhang2, E J McGhee1, C B Talbot1, J McGinty1, T D Bunney2, I Munro1, C Dunsby1, P Courtney3, M Katan2, M A A Neil1, and P M W French1.
1 Dept of Physics, Imperial College London, UK; 2 Div Cell. Endovenously administered oncolytic viruses extravasate and penetrate poorly into tumors. iRGD is a cyclic peptide that enhances tumor penetration when conjugated or.
Accelerated optical projection tomography applied to in vivo imaging of zebrafish, PLOS One, Vol: 10, ISSN: Optical projection tomography (OPT) provides a non-invasive 3-D imaging modality that can be applied to longitudinal studies of live disease models, including in zebrafish.
Imaging in turbid media: a transmission detector gives order of magnitude enhanced sensitivity compared to epi-detection schemes. Biomed Opt Express. ; 7 (9): pIRES2-EGFP IRES-containing bicistronic vector for expressing a gene together with EGFP.
Detection of orthotopic xenograft tumors is difficult due to poor spatial resolution and reduced image fidelity with traditional optical imaging modalities. In particular, light scattering and attenuation in tissue at depths beyond subcutaneous implantation hinder adequate visualization.
We evaluate the use of multispectral optoacoustic tomography (MSOT) to detect upregulated epidermal. Cell 23, Issue 1 pe6, 3 April / DNA damage repair (DDR) pathways modulate cancer risk, progression and therapeutic response.
We systematically analyzed somatic alterations across 33 cancer types in order to provide a comprehensive view of DDR deficiency in cancer. The role of endocytosis in the control of EGF receptor (EGFR) activation and cell signaling was explored by using mouse fibroblasts in which dynamin was conditionally depleted.
Dynamin is a GTPase shown to play an important role in the control clathrin mediated endocytosis of EGFR and other cell surface receptors. In this report, we demonstrate that EGF binding activity and the display of high.